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We’re in the process of biobllock new support for the specification of devices in the Registry. For the time being, please see the existing device tables below. See more info on Registry collections. Unless otherwise specified, most parts in the Registry catlogue with the original BioBrick assembly standard also known as Assembly standard These pages have not undergone curation by the Registry but have been made by the Registry user community. Please feel free to add new catalog pages to this section.
Over time, high quality pages will be “promoted” to the primary Registry catalog collection. A promoter is a DNA sequence that tends to recruit transcriptional machinery and lead to transcription of the downstream DNA sequence. Protein domains are portions of proteins cloned in frame with other proteins domains to make up a protein coding sequence.
Some protein domains might change the protein’s location, alter its degradation rate, target the protein for cleavage, or enable it to be readily purified.
Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon. Translational units are composed of a ribosome binding site and a protein coding sequence.
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They begin at the site of translational initiation, the RBS, and end at the site of translational termination, the stop codon. A terminator is an RNA sequence that usually occurs at the end of a gene or operon mRNA and causes transcription to stop.
DNA parts include cloning sites, scars, primer binding sites, spacers, recombination catalgue, conjugative tranfer elements, transposons, origami, and aptamers.
A plasmid is a circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. A plasmid backbone is defined as the plasmid sequence beginning with the BioBrick suffix, including the replication origin and antibiotic resistance marker, and ending with the BioBrick prefix.
If you’re looking for a plasmid or vector to propagate or assemble plasmid backbones, please see the set of plasmid backbones. There are a few parts in the Registry that are only available as circular plasmids, not as parts in a plasmid backbone, you can find them here.
Note that these plasmids largely do not conform to the BioBrick standard.
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Although primers are not actually available via the Registry distribution, we include commonly used primer sequences here. Composite parts are combinations of of two or more BioBrick parts. Parts and devices improving biological containment. Parts involved in the production or degradation of chemicals and metabolites are listed here. Cell-cell signaling and quorum sensing: Cataloge involved in intercellular signaling and quorum sensing catalovue bacteria.
Parts involved in killing cells. Parts involved in taking a bacterial photograph. Parts involved in DNA conjugation between bacteria.
Parts involved in motility or chemotaxis of cells. Odor production and sensing: Parts the produce or sense odorants. Parts involved in DNA recombination. Parts involved in the production and modification of Viral vectors. Most parts in the Registry function in E. Yeast are simple eukaryotes. Bacteriophage T7 is an obligate lytic phage of E.
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Bacillus subtilis is a model gram-positive bacterium. There are now dozens of MammoBlocks suitable for rapid expression in mammalian cells. Assembly standard 10, or the original BioBrick assembly standard, was developed by Tom Knight in Most parts in the Registry comply with this assembly standard.
Assembly standard 23, or the Silver standard, is compatible with original BioBrick assembly standard and allows for in-frame assembly of protein domains. Assembly standard 25, or the Freiburg standard, extends upon the original BioBrick assembly standard and allows for in-frame assembly of protein domains. Assembly standard 21, also known as the BglBrick, BBb, or Berkeley standard, is optimized to enable in-frame assembly of protein domains.
Assembly standard 28, also known as the Lim lab standard or AarI cloning, is optimized for assembly of 3 parts into a vector simultaneously. Most parts that comply with Assembly standard 28 function in yeast. Julie Norville has developed a new set of parts for assembly of fusion proteins. Most parts in the Registry operate in E.
Mesoplasma florum is a particularly simple model organism. The miniTn7 BioBrick tool kit is a set of fully BioBrick-compatible vectors based catlaogue Tn7 transposon for the integration of parts into microbial genomes.