IRWIN FRIDOVICH. The Department of Biochemistry, Duke University Medical Center,. Durham, North Carolina Received April 21, Superoxide. Beauchamp, C. and Fridovich, I. () Superoxide Dismutase Improved Assays and an Assay Applicable to Acrylamide Gels. Analytical Biochemistry, Author: Beauchamp C, Journal: Analytical biochemistry[/11] Fridovich I. Find all citations in Analytical Biochemistry [01 Nov , 44(1)]. /.
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Analytical Biochemistry, 44, Advances in Enzyme ResearchVol. The reduction of nitroblue tetrazolium by superoxide radicals generated from photo-reactive riboflavin has been in use for more than four decades to detect superoxide dismutase SOD on nondenaturing polyacrylamide gels.
SOD research in medicine and biochemistry has warranted the development of multiple assay variants to overcome specific experimental constraints or to combine the SOD assay with other enzyme assays. Fine-tuning reagent concentrations to effectively visualize bands continue to be a major research obstacle in assay development.
Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.
Herein we describe a straightforward technique to reliably adjust the background color of polyacrylamide gels without compromising assay efficacy. Low micromolar to low millimolar concentrations of yellow riboflavin can be mixed with the blue of reduced nitroblue tetrazolium to controllably produce blue, purple, yellow-brown, or yellow gel backgrounds. The advantage of this technique is that the assay is not modified by the introduction of new reagents. Quantitative reliability of these alternative stains was assessed by plotting determined band intensity values against known enzyme loads.
The correlation R2 values of trial averages were compared against the average correlation of the standard 0.
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Assay sensitivity was assessed by comparing lowest possible visible enzyme load of the experimental stains with the 0. No difference in the quantitative reliability fridovuch found in any riboflavin concentration. The minimum reliable sensitivity of the assay was found to be 10 ng for each concentration of riboflavin. This technique has already been employed to analyze SOD protein expression levels in extracts of Escherichia coli Bertrand et al.
Budi Handono, Firman F. Mose, Tri Hanggono Achmad. Yan Li, Nathaniel P. Glutathione peroxidase, superoxide dismutase and catalase activities in children with chronic hepatitis. Nagwa Abdallah Ismail, Sawsan H. Okasha, Anil Dhawan, Azza M.